This study was conducted to examine the effect of magnesium consumption on electromyography indexes of muscle fatigue after intense anaerobic exercise. The participants in this experiment were 16 volunteers of college-aged males. These subjects were divided randomly into two supplement and placebo groups. The electrodes of electromyography for the muscles were located according to the SENIAM procedure. The test was carried out on ergometric bicycles (Lode, made in the Netherlands) and the muscles’ activity of the right leg was recorded by an EMG device, simultaneously. Independent and paired t-test were used to identify inter and intragroup differences. The significance level of the tests was assumed as 0.05. Consuming magnesium supplement reduced RMS of interior and external wide muscles and interior twin muscles (P=0.001, P=0.05, P=0.05, respectively), and increased MPF of interior and external wide muscles and interior twin muscles (P=0.001, P=0.05, P=0.05, respectively). According to the findings of the present study, utilizing magnesium sulfate supplement about 350 mg can be prescribed to enhance the performance of healthy subjects in severe short-term activities, i.e. Wingate test.